Yeastlink-Primer Design

Primer Design

Template DNA Sequence:


Target Start Position (optional, 0-based):


Target End Position (optional, 0-based):


Upstream Overhang (5' end):


Downstream Overhang (3' end):


Minimum Primer Length (annealing part):


Maximum Primer Length (annealing part):


Minimum Tm (°C) for Annealing Part:


Design Primers

Score a Primer (Single Template)

Primer Sequence:


Template DNA Sequence:


Paired Primer (optional):


Score Primer